ELISA Kit DIY Materials for Coagulation Factor VII (F7)
FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSB874Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryMetabolic pathwayInfection immunityHematology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SamplePlasma.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range78-5,000pg/mL
- Applicable Sensitivity33pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Coagulation Factor VII (F7). No significant cross-reactivity or interference between Coagulation Factor VII (F7) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture
Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Monocytes regulate systemic coagulation and inflammation in abdominal sepsisPubmed:25502108
- Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testingPubMed: 26243277
- Loss of accelerates arterial thrombosis by increasing formation of neutrophil extracellular traps and plasma tissue factor activityPubmed:29444200