ELISA Kit DIY Materials for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Homo sapiens (Human) Assay Kit DIY Materials

CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1000pg/mL
  • Applicable Sensitivity5.9pg/mL
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  • ELISA Kit DIY Materials for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Packages (Simulation)
  • ELISA Kit DIY Materials for Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF). No significant cross-reactivity or interference between Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic SystemPubMed: 20391130
  • Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trialPubmed:24525316
  • Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central ChinaPubmed:29305646
  • Activated Macrophages of Monocytic Origin Predominantly Express Proinflammatory Cytokine Genes, Whereas Kupffer Cells Predominantly Express Anti …
  • Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal OriginPubmed: 31183654
  • The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­33285209
  • Co-delivery of PSMA antigen epitope and mGM-CSF with a cholera toxin-like chimeric protein suppressed prostate tumor growth via activating dendritic cells and?¡­33612342

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