ELISA Kit DIY Materials for Complement C3 Convertase (C3 Convertase)
C4b2a; C3bBb, complement C.hivin.4.hivin2
- UOM
- FOB US$ 1,008.00 US$ 1,680.00 US$ 3,024.00 US$ 5,880.00 US$ 10,080.00
- Quantity
Overview
Properties
- Product No.KSA758Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transductionInfection immunityImmune molecule
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.78-50ng/mL
- Applicable Sensitivity0.31ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Complement C3 Convertase (C3c).No significant cross-reactivity or interference between Complement C3 Convertase (C3c) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Circulation levels of acute phase proteins in patients with Takayasu arteritisPubMed: 20100644
- Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitisPubmed:27235358
- Leishmania donovani Inhibitor of Serine Peptidases 2 Mediated Inhibition of Lectin Pathway and Upregulation of C5aR Signaling Promote Parasite Survival …Pubmed:29434593