ELISA Kit DIY Materials for Complement Component 3a (C3a) Rattus norvegicus (Rat) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids。
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range6.25-400pg/mL
  • Applicable Sensitivity2.81pg/mL
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  • ELISA Kit DIY Materials for Complement Component 3a (C3a) Packages (Simulation)
  • ELISA Kit DIY Materials for Complement Component 3a (C3a) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Complement Component 3a (C3a). No significant cross-reactivity or interference between Complement Component 3a (C3a) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Immobilization of soluble complement receptor 1 on islets.ScienceDirect: S0142961211002596
  • Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsiaPubMed: 23415845
  • Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis modelPubmed: 23645587
  • Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in ratsPubmed: 24353977
  • Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia?PubMed: 26485247
  • Human IgG Antinuclear Antibodies Induce Pregnancy Loss in Mice by Increasing Immune Complex Deposition in Placental Tissue: In Vivo StudyPubMed: 26388133
  • Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitisPubmed:27235358
  • Alternative Pathway Inhibition by Exogenous Factor H Fails to Attenuate Inflammation and Vascular Leakage in Experimental Pneumococcal Sepsis in MicePubmed:26872035
  • Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos10183
  • Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns.pubmed:28131092
  • Accuracy of complement activation product levels to detect infected pleural effusion in ratspubmed:28474458
  • The Complement C3a–C3aR Axis Promotes Development of Thoracic Aortic Dissection via Regulation of MMP2 ExpressionPubmed:29367209
  • Long term safety and immunological effects of a nanobiotherapeutic, bovine poly-[hemoglobin-catalase-superoxide dismutase-carbonic anhydrase], after four weekly …Pubmed:29873524
  • Mesenchymal stromal cell secretory factors induce sustained improvements in islet function pre-and post-transplantationDoi: 10.1016/j.jcyt.2018.07.007
  • Intrathecal complement activation by the classical pathway in tick-borne encephalitisPubmed: 30850976
  • Pathogenic roles of anti-C1q antibodies in recurrent pregnancy loss
  • Hemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys
  • Complement-mediated hypersensitivity reactions to an amphotericin B-containing lipid complex (Abelcet) in pediatric patients and anesthetized rats: Benefits of slow infusion33549818
  • Increased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression33493474
  • C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney diseasePubmed:35503088

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