ELISA Kit DIY Materials for Complement Factor D (CFD)
CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSB833Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryImmune molecule
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.47-30ng/mL
- Applicable Sensitivity0.22ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Complement Factor D (CFD). No significant cross-reactivity or interference between Complement Factor D (CFD) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Relationship between serum adipsin and the first phase of glucose‐stimulated insulin secretion in individuals with different glucose tolerancePubmed:29432659
- CORRELATION OF INCREASED SERUM ADIPSIN WITH INCREASED CARDIOVASCULAR RISKS IN ADULT PATIENTS WITH GROWTH HORMONE DEFICIENCYPubmed: 30657359
- Association between plasma adipsin level and mild cognitive impairment in Chinese patients with type 2 diabetes: a cross-sectional studyPubmed: 31651303
- HFD-induced hepatic lipid accumulation and inflammation are decreased in Factor D deficient mousePubmed: 33067533
- Body fat distribution and circulating adipsin are related to metabolic risks in adult patients with newly diagnosed growth hormone deficiency and improve after …Pubmed: 33254428
- Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis33367879