ELISA Kit DIY Materials for Creatine Kinase, Muscle (CKM)

CK-MM; CKMM; CK3; CK-M; MM-CK; M-CK; Creatine kinase M-type, N-terminally processed

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,166.00 US$ 1,944.00 US$ 3,499.00 US$ 6,804.00 US$ 11,664.00
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Overview

Properties

Product No.KSA109Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase Cardiovascular biology

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range1.56-100ng/mL
Applicable Sensitivity0.63ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Creatine Kinase, Muscle (CKM). No significant cross-reactivity or interference between Creatine Kinase, Muscle (CKM) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Assessment of endothelium and inflammatory response at the onset of reperfusion injury in hand surgeryPubMed: 22583529
Extraocular Muscle Characteristics Related to Myasthenia Gravis SusceptibilityPubMed: PMC3568149
Fructokinase activity mediates dehydration-induced renal injuryPubmed: 24336030
Methylxanthine induced cardiotoxicity and its mechanisms: An experimental study230b:34bf146150b42971d76dda5e8329bc5f2f0b.pdf
Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusionPubmed:29393473
Proteomic and Metabolomic Profiling Identify Plasma Biomarkers for Exposure to Ultra-low Levels of CarfentanilPubmed: 30321427
The effects of human sera conditioned by high-intensity exercise sessions and training on the tumorigenic potential of cancer cellsPubmed: 32447643