ELISA Kit DIY Materials for Cyclophilin A (CYPA)
PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSA979Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryTumor immunityInfection immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.064ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Cyclophilin A (CYPA).No significant cross-reactivity or interference between Cyclophilin A (CYPA) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formationPubMed: 19758589
- Cyclophilin-A: a novel biomarker for untreated male essential hypertensionPubmed: 24164594
- Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular diseasePubmed:24502618
- Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial InfarctionPubmed:25552928
- Cyclophilin A in Ruptured Intracranial AneurysmPubMed: 26426668
- PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery diseasePubMed: 26702934
- Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes MellitusPubMed: 26496315
- Cyclophilin A modulates bone marrow-derived CD117(+) cells and enhances ischemia-induced angiogenesisvia the SDF-1/CXCR4 axis.Pubmed:27057951
- Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitorpubmed:27823952
- Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus.pubmed:27809851
- Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activityPubmed:29382697
- Cyclophilin a increases CD68 cell infiltration in rat experimental periodontitisPubmed:29318411
- Cyclophilin A and CD147 associate with progression of diabetic nephropathyPubmed: 30572748
- Association of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarctionPubmed: 31670477
- Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome PatientsPubmed: 31936351
- APPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers …Pubmed: 24030206
- Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy
- Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy34437430
- Proteomic analysis reveals USP7 as a novel regulator of palmitic acid-induced hepatocellular carcinoma cell deathPubmed:35732625