ELISA Kit DIY Materials for Endoglin (ENG)
CD105; END; HHT1; ORW; ORW1; Osler-Rendu-Weber Syndrome 1
- UOM
- FOB US$ 896.00 US$ 1,494.00 US$ 2,689.00 US$ 5,229.00 US$ 8,964.00
- Quantity
Overview
Properties
- Product No.KSA980Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryTumor immunityInfection immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.062ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Endoglin (ENG).No significant cross-reactivity or interference between Endoglin (ENG) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the LiteraturePubMed: 26142066
- Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkersscience:S1646706X16300052
- Impaired angiogenesis as a feature of digital ulcers in systemic sclerosisPubmed:26920752
- Diagnostic and prognostic significance of serum soluble endoglin levels in preeclampsia and eclampsiapubmed:27563629
- Relationships between serum selenium and zinc concentrations versus profibrotic and proangiogenic cytokines (FGF-19 and endoglin) in patients with alcoholic liver cirrhosis.pubmed:28954507
- The predictive role of PlGF/sENG ratio in fetal growth restriction of early beginning associated with pre-eclampsia