ELISA Kit DIY Materials for Enolase, Neuron Specific (NSE) Homo sapiens (Human) Assay Kit DIY Materials

ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.625-40ng/mL
  • Applicable Sensitivity0.231ng/mL
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  • ELISA Kit DIY Materials for Enolase, Neuron Specific (NSE) Packages (Simulation)
  • ELISA Kit DIY Materials for Enolase, Neuron Specific (NSE) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Enolase, Neuron Specific (NSE). No significant cross-reactivity or interference between Enolase, Neuron Specific (NSE) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in RatsLiebert: 20091210
  • Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladderWiley: source
  • Ginkgo biloba Enhances the Anticonvulsant and Neuroprotective Effects of Sodium Valproate Against Kainic Acid-induced Seizures in MiceMedwell: 2011679690
  • Comparative gene expression profiling analysis of lymphoblastoid cells reveals neuron‐specific enolase gene (ENO2) as a susceptibility gene of heroin dependencePubMed: 21995595
  • Advanced glycation end products as an upstream molecule triggers ROS-induced sFlt-1 production in extravillous trophoblasts: A novel bridge between oxidative stress and preeclampsiaPubmed: 24144948
  • Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats.Pubmed: 24291152
  • Detection of β-amyloid oligomers as a predictor of neurological outcome after brain injury.Pubmed: 23540266
  • A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer read outRsc: Source
  • The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbitsPubmed:25111247
  • Elevated levels of serum sICAM-1 in asphyxiated low birth weight newbornsPubmed:25358349
  • Preconditioning effect of (S)-3,5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague-Dawley rats.Pubmed:25562631
  • 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究Journal: Fulltext
  • Blood–Brain Barrier Function and Biomarkers of Central Nervous System Injury in Rickettsial versus Other Neurological Infections in LaosPubMed: 26055741
  • Preconditioning effect of (S)-3, 5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague–Dawley ratsPubMed: 25562631
  • S100B and NSE serum concentrations after simulated diving in ratsPubMed: 26462746
  • The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesionsPubMed: 26184156
  • Isoflurane Post-Treatment Improves Outcome after an Embolic Stroke in RabbitsPubMed: 26645542
  • Волгоградский государственный медицинский университетuploads:dsovet
  • Synthesis of sunflower-like gold nanostructures and their application in electrochemical immunoassays using the nanogold-triggered hydrogen evolution reactionarticlelanding:c5ay03174e
  • 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响article:9794
  • Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injuryscience:S1995764516300955
  • SERUMSKA RAZINA ZA NEURON SPECIFIČNE ENOLAZE KAO BILJEG ISHEMIJSKO-REPERFUZIJSKOG OŠTEĆENJA U BOLESNIKA PODVRGNUTIH KAROTIDNOJ ENDARTEREKTOMIJI262238
  • Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acidpubmed:27490041
  • Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in ratspubmed:28076830
  • Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β …pubmed:29040978
  • Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathwayPubmed:29510144
  • Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling PathwayPubmed:29770336
  • S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid …Pubmed:29801873
  • Nitrosative stress indices in dogs with neurological form of canine distemperDoi: 10.22099/IJVR.2018.4943
  • Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway
  • Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema
  • The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the …
  • Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion …Pubmed: 32214270
  • Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 …Pubmed: 32300366
  • Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and …Pubmed: 32432773
  • Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats
  • The role of light desynchronosis in the development of stress-induced aging
  • ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ …
  • Investigation of Library Input on Aptamers Selection Efficiency Using Capillary Electrophoresis
  • The effect of doxycycline on neuron-specific enolase in patients with traumatic brain injury: a randomized controlled trial34262678
  • Perillaldehyde Alleviates Spinal Cord Ischemia-Reperfusion Injury Via Activating the Nrf2 Pathway34399353
  • Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome34644195

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