ELISA Kit DIY Materials for Factor Related Apoptosis Ligand (FASL) Homo sapiens (Human) Assay Kit DIY Materials

CD178; CD95L; CD95-L; FASLG; FASLG; APT1LG1; APT1-LG1; TNFSF6; Fas Antigen Ligand; TNF Superfamily Member 6; Tumor Necrosis Factor(ligand)Superfamily,Member 6

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000pg/mL
  • Applicable Sensitivity6.5pg/mL
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  • ELISA Kit DIY Materials for Factor Related Apoptosis Ligand (FASL) Packages (Simulation)
  • ELISA Kit DIY Materials for Factor Related Apoptosis Ligand (FASL) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Factor Related Apoptosis Ligand (FASL).No significant cross-reactivity or interference between Factor Related Apoptosis Ligand (FASL) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Macrophage-expressed IFN-β Contributes to Apoptotic Alveolar Epithelial Cell Injury in Severe Influenza Virus PneumoniaPubMed: PMC3585175
  • The diplotype Fas? 1377A/? 670G as a genetic marker to predict a lower risk of breast cancer in Chinese womenSpringer:Source
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA

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