ELISA Kit DIY Materials for Fatty Acid Binding Protein 2, Intestinal (FABP2) Rattus norvegicus (Rat) Assay Kit DIY Materials

FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.133ng/mL
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  • ELISA Kit DIY Materials for Fatty Acid Binding Protein 2, Intestinal (FABP2) Packages (Simulation)
  • ELISA Kit DIY Materials for Fatty Acid Binding Protein 2, Intestinal (FABP2) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 2, Intestinal (FABP2).No significant cross-reactivity or interference between Fatty Acid Binding Protein 2, Intestinal (FABP2) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Cranberries (Oxycoccus quadripetalus) inhibit lipid metabolism and modulate leptin and adiponectin secretion in 3T3-L1 adipocytesPubMed: 25952883
  • Elevation of HO-1 Expression Mitigates Intestinal Ischemia-Reperfusion Injury and Restores Tight Junction Function in a Rat Liver Transplantation ModelPubMed: 26064429
  • Immunomodulatory potential of β-glucan as supportive treatment in porcine rotavirus enteritis.pubmed:28895864
  • Protective effect of aplysin on liver tissue and the gut microbiota in alcohol-fed ratspubmed:28622357
  • Effect of Porcine Rotavirus Enteritis on Concentrations of Interferonγ, Immunoglobulin-G and Intestinal Fatty Acid-Binding Protein-2 and Peripheral Leukocyte Function10.5958/0973-9149.2017.00011.9
  • Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory …Pubmed:29672286
  • Lack of liver steatosis in germ-free mice following hypercaloric dietsPubmed:29926176
  • Protective Effect of Aplysin Supplementation on Intestinal Permeability and Microbiota in Rats Treated with Ethanol and IronPubmed:29861488
  • Intestinal fatty acid binding protein (I-FABP) as a marker for acute intestinal ischemiaDoi: 10.18203/2349-2902.isj20190058
  • Effect of a Multistrain Probiotic on Cognitive Function and Risk of Falls in Patients With Cirrhosis: A Randomized Trial
  • Immunomodulating dose of levamisole stimulates innate immune response and prevents intestinal damage in porcine rotavirus diarrhea: a restricted-randomized …Pubmed: 30790158
  • Role of Aquaporin-3 in Intestinal Injury Induced by SepsisPubmed: 31582652
  • FABP1 and FABP2 as markers of diabetic nephropathyPubmed: 32922200
  • Hydrogen attenuates radiation-induced intestinal damage by reducing oxidative stress and inflammatory responsePubmed: 32361189
  • Markers of Intestinal Damage in Individuals with and without Obesity during a 12-Week Exercise Period.
  • Effects of fucoidan on tumor prevention and gut flora
  • Effect of Lactobacillus casei on lipid metabolism and intestinal microflora in patients with alcoholic liver injury33514869
  • The ¦Á2AR/Caveolin©\1/p38MAPK/NF©\¦ÊB axis explains dexmedetomidine protection against lung injury following intestinal ischaemia©\reperfusion34114328
  • Somatization in patients with predominant diarrhoea irritable bowel syndrome: the role of the intestinal barrier function and integrity34022802
  • Impact of Successive Exertional Heat Injuries on Thermoregulatory and Systemic Inflammatory Responses in Mice34528459
  • The protective effects of HIF-1α activation on sepsis induced intestinal mucosal barrier injury in rats model of sepsisPubmed:35576220

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