ELISA Kit DIY Materials for Ferritin (FE)
- UOM
- FOB US$ 716.00 US$ 1,194.00 US$ 2,149.00 US$ 4,179.00 US$ 7,164.00
- Quantity
Overview
Properties
- Product No.KSA518Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - DownloadInstruction Manual
- CategoryMetabolic pathwayTumor immunityHematology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.068ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Ferritin (FE). No significant cross-reactivity or interference between Ferritin (FE) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation.Pubmed: 23438894
- Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma.PubMed: 25804229
- Host H68D Genotype Affects Tumor Growth in Mouse MelanomaOpen-Access: 1948-5956-1000353
- The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing micePubmed:26757699
- The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Diseasepubmed:27782743
- 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain.pubmed:27615544
- Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activationpubmed:29147463
- sucrosomial® iron absorption studied by in vitro and ex-vivo modelspubmed:29055735
- In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells.pubmed:29051659
- Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women10.1590/MEDICC.2017.1902030004
- The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus.pubmed:28584599
- Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system10.1016/j.fbio.2017.10.001
- Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Ratspubmed:28629118
- Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cellsPubmed:29622185
- Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet10.1016:j.anifeedsci.2018.01.012
- Iron homeostasis in pregnancy and spontaneous abortionPubmed: 30394565
- Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria
- New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemiaPubmed: 30792208
- PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE
- Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic
- Transferrin plays a central role in coagulation balance by interacting with clotting factorsPubmed: 31811276
- Gum arabic capped copper nanoparticles: Synthesis, characterization, and applicationsPubmed: 31904465
- Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State FermentationPubmed: 32255407
- Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability
- Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure34139233
- The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11Pubmed:35700835