ELISA Kit DIY Materials for Galectin 1 (GAL1)
LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin
- UOM
- FOB US$ 1,361.00 US$ 2,268.00 US$ 4,082.00 US$ 7,938.00 US$ 13,608.00
- Quantity
Overview
Properties
- Product No.KSA321Bo01
- Organism SpeciesBos taurus; Bovine (Cattle) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryTumor immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range78.12-5000pg/mL
- Applicable Sensitivity27.5pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Galectin 1 (GAL1).No significant cross-reactivity or interference between Galectin 1 (GAL1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: Regulatory roles of cell surface glycansPubmed:24589677
- Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk populationPubmed:25578395
- Cord blood galectin-1 and-3 concentrations in term pregnancies with normal restricted and increased fetal growthPubMed: 24717333
- Noninvasive small-animal imaging of galectin-1 upregulation for predicting tumor resistance to radiotherapyPubmed:29272764
- Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary DiseasePubmed:29429028
- Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosisPubmed: 31678459
- Galectin expression detected by 68Ga-galectracer PET as a predictive biomarker of radiotherapy resistancePubmed:35106644
- Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive …Pubmed:35687771
- In Vitro Bovine Embryo Production Using Medium's Supplemented with Recombinant Human Galectin-1 Buffered