ELISA Kit DIY Materials for Gelsolin (GSN)
GS; AGEL; ADF; Brevin; Gelsolin Amyloidosis,Finnish Type; Actin-depolymerizing factor
- UOM
- FOB US$ 1,008.00 US$ 1,680.00 US$ 3,024.00 US$ 5,880.00 US$ 10,080.00
- Quantity
Overview
Properties
- Product No.KSA372Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryTumor immunityInfection immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range62.5-4000pg/mL
- Applicable Sensitivity28.2pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Gelsolin (GS). No significant cross-reactivity or interference between Gelsolin (GS) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Gelsolin in Cerebrospinal Fluid as a Potential Biomarker of EpilepsySpringerLink: 3585r8h46q7r0171
- Treatment with gelsolin reduces brain inflammation and apoptotic signaling in mice following thermal injuryBioMed: 17422094
- Application of iTRAQ quantitative proteomics in identification of serum biomarkers in breast cancerIEEE: 6098563
- Investigation of the actin scavenging system in pre-eclampsiaPubmed: 24239294
- Proteomic Study Reveals Plasma Protein Changes in Congenital Heart DiseasesPubmed: 24565402
- The role of plasma gelsolin in cardiopulmonary bypass induced acute lung injury in infants and young children: a pilot studyPubmed:Pmc4132929
- Gelsolin: Role of a Functional Protein in Mitigating Radiation InjuryPubmed:25164111
- Plasma gelsolin and matrix metalloproteinase 3 as potential biomarkers for Alzheimer diseasePubMed: 25864780
- Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patientsPubmed:26680509
- Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.pubmed:26680509
- Serum gelsolin levels in aneurismal subarachnoid hemorrhage: Preliminary results.pubmed:28365030
- Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental MethodsPubmed:30040720
- Plasma gelsolin level predicts acute kidney injury after cardiopulmonary bypass in infants and young childrenPubmed:29427164
- Production and Secretion of Gelsolin by Both Human Macrophage-and Fibroblast-like Synoviocytes and GSN Modulation in the Synovial Fluid of Patients with Various …Pubmed:35327525