ELISA Kit DIY Materials for Glial Fibrillary Acidic Protein (GFAP) Rattus norvegicus (Rat) Assay Kit DIY Materials

Intermediate Filament Protein

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range62.5-4000pg/mL
  • Applicable Sensitivity24.2pg/mL
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  • ELISA Kit DIY Materials for Glial Fibrillary Acidic Protein (GFAP) Packages (Simulation)
  • ELISA Kit DIY Materials for Glial Fibrillary Acidic Protein (GFAP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Glial Fibrillary Acidic Protein (GFAP). No significant cross-reactivity or interference between Glial Fibrillary Acidic Protein (GFAP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat BrainPubmed: 23183024
  • EFECTO PROTECTOR DEL TRATAMIENTO CON NEUROEPO SOBRE LA GLIA DE LA CORTEZA CEREBRAL EN RATONES TRANSGéNICOS CON LA ENFERMEDAD DE ALZHEIMER.Sld:Source
  • Effect of memantine, a NMDA receptor blocker, on ethambutol-induced retinal injuryPubMed: 26704355
  • IL-4 Inhibits IL-1β-Induced Depressive-Like Behavior and Central Neurotransmitter AlterationsPubMed: 26417153
  • Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the RatPubMed: 26700436
  • Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s DiseasePubMed: 26119304
  • Relationship of orexin (hypocretin) system and astrocyte activation in Parkinson's disease with hypersomnolenceDoi: 10.1111
  • Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s DiseasePubmed:26119304
  • Effect of memantine: A NMDA receptor blocker, on ethambutol-induced retinal injuryPubmed:26704355
  • ESTROGENIC COMPONENTS FOR USE IN THE TREATMENT OF NEUROLOGICAL DISORDERSy2016:0101116.html
  • Human plasma glial fibrillary acidic protein and heat shock protein 27 concentrations in acute moderate-intensity aerobic exercise with different durationa825e1e27b0221df9a627769c0f614432e92.pdf
  • Comparison of GFAP and HSP27 concentrations in acute moderate-intensity aerobic exercise of different duration2031884
  • Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms.pubmed:27777064
  • Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms.pubmed:27915041
  • Troxerutin exerts neuroprotection in 6-hydroxydopamine lesion rat model of Parkinson's disease: Possible involvement of PI3K/ERβ signaling.pubmed:28284752
  • Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat.pubmed:28199883
  • Estrogen receptors and estetrol-dependent neuroprotective actions: a pilot study.pubmed:27799463
  • An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's …articles:journal-of-alzheimers-disease
  • Evaluierung von Biomarkern im Liquor cerebrospinalis bei paraplegischen Hunden mit Rückenmarksverletzung:
  • Utilization of Glial Fibrillary Acidic Protein and Galectin-3 in the Diagnosis of Cerebral Infarction Patients with Normal Cranial Tomographypubmed:28406123
  • Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effectspubmed:29102164
  • Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanismspubmed:28061403
  • The anti-aging protein klotho alleviates injury of nigrostriatal dopaminergic pathway in 6-hydroxydopamine rat model of Parkinson's disease: Involvement of PKA/CaMKII/CREB signaling.pubmed:29107062
  • Acrolein-mediated neurotoxicity in growing Wistar male ratsPubmed:30033014
  • Mechanisms of death in structurally normal stillbirthsPubmed: 30231013
  • Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanismsPubmed: 30456649
  • Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanismsPubmed: 30421246
  • INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA
  • The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the …
  • Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic RetinopathyPubmed: 32165929
  • Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying MechanismsPubmed: 32526752
  • Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairmentPubmed: 28593326
  • Quercetin mitigates monosodium glutamate-induced excitotoxicity of the spinal cord motoneurons in aged rats via p38 MAPK inhibitionPubmed: 32622428
  • The Predictive Role of Neurobiochemical Markers in Multiple Sclerosis.
  • Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease33995926
  • PHARMACOLOGICAL SCREENING OF A POTENTIALLY EFFECTIVE COMPOUND FOR THE TREATMENT OF CHRONIC TRAUMATIC ENCEPHALOPATHY?¡­
  • The bee venom active compound melittin protects against bicuculline-induced seizures and hippocampal astrocyte activation in rats34808488
  • P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord InjuryPubmed:35154311

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