ELISA Kit DIY Materials for Growth Differentiation Factor 15 (GDF15)
PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor
- UOM
- FOB US$ 1,260.00 US$ 2,100.00 US$ 3,780.00 US$ 7,350.00 US$ 12,600.00
- Quantity
Overview
Properties
- Product No.KSC034Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryCytokineApoptosisTumor immunityInfection immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.069ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Growth Differentiation Factor 15 (GDF15).No significant cross-reactivity or interference between Growth Differentiation Factor 15 (GDF15) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Growth Differentiation Factor 15 and Coronary Collateral FormationPubMed: 20014173
- Elevated levels of GDF-15 is associated with increased angiotensin II in hypertensive patients with Type 2 diabetespme-2016-0030
- Rapamycin improves renal injury induced by Iodixanol in diabetic rats by deactivating the mTOR/p70S6K signaling pathwayPubmed: 32798557
- Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort
- Serum NT©\proBNP levels are associated with cognitive functions in hemodialysis patients33423325
- Is GDF-15 level associated with gestational diabetes mellitus and adverse perinatal outcomes?33678319
- Changes in Plasma Growth Differentiation Factor-15 After Laparoscopic Sleeve Gastrectomy in Morbidly Obese Patients: A Prospective Study33880052
- Predictive value of growth differentiation factor-15 in patients with myocardial infarction
- High Fat, High Sugar Diet and DJOS Bariatric Surgery Influence Plasma Levels of Fetuin-B, Growth Differentiation Factor-15, and Pentraxin 3 in Diet-Induced Obese …34684637
- Small extracellular vesicle-mediated targeting of hypothalamic AMPKα1 corrects obesity through BAT activation34675439
- Growth Differentiation Factor 15 is Related with Left Ventricular Recovery in Patients with ST-Elevation Myocardial Infarction after Successful Reperfusion by …34584380
- The effect of bariatric surgery on the expression of Growth Differentiation Factor-15/Macrophage-Inhibitory Cytokine-1 (GDF-15/MIC-1) in ratPubmed:35029766
- Plasma growth differentiation factor‐15 in patients with “lone” atrial fibrillationPubmed:35334497