ELISA Kit DIY Materials for Heat Shock 70kDa Protein 1A (HSPA1A)

HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
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Overview

Properties

Product No.KSB081Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategorySignal transduction Tumor immunity Infection immunity Developmental science

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates and other biological fluids
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.781-50ng/mL
Applicable Sensitivity0.28ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 1A (HSPA1A).No significant cross-reactivity or interference between Heat Shock 70kDa Protein 1A (HSPA1A) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

Identification of potential biomarkers of gold nanoparticle toxicity in rat brainsPubMed: 22691312
Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in PigsPubmed:Pmc4324980
Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in ratsPubmed:24508522
Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing micePubmed:25443743
A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmaticspubmed:28822267
Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young …Pubmed:29379533
Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampusPubmed: 32172517
Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in micePubmed: 32439493