ELISA Kit DIY Materials for Hemojuvelin (HJV)
HFE2; HFE2A; HJV; JH; RGMC; hemochromatosis type 2(Juvenile); Haemojuvelin; RGM domain family member C
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSB995Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryMetabolic pathwayInfection immunityHematologyNutrition metabolismHormone metabolism
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.625-40ng/mL
- Applicable Sensitivity0.256ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Hemojuvelin (HJV).No significant cross-reactivity or interference between Hemojuvelin (HJV) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Effect of Iron Overload and Iron Deficiency on Liver Hemojuvelin ProteinPubMed: PMC3356351
- Hepcidin Levels Are Increased in Patients with Acute Ischemic Stroke: Preliminary ReportPubMed: 25881778
- Soluble hemojuvelin in transfused and untransfused thalassaemic subjectsdoi:10.1111
- The secret war against counterfeit sciencepubmed:28492262
- Urinary biomarkers predict advanced acute kidney injury after cardiovascular surgeryPubmed:29699579
- Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac SurgeryPubmed:29386545
- The functional role of hemojuvelin in acute ischemic strokePubmed: 31307288
- Oxidative damage and iron dyshomeostasis in thalassemic patients
- Caffeine and alcohol-friends or foes of human iron stores?Pubmed:35063815
- Urinary Biomarkers Can Predict Weaning From Acute Dialysis Therapy in Critically Ill PatientsPubmed:35311928
- The Relationships among Plasma Fetuin-B, Thyroid Autoimmunity, and Fertilization Rate In Vitro Fertilization and Embryo TransferPubmed:35340679