ELISA Kit DIY Materials for Immunoglobulin G3 (IgG3) Homo sapiens (Human) Assay Kit DIY Materials

IGHG3; HDC; Heavy chain disease protein; Ig Gamma-3 Chain C Region; Immunoglobulin Heavy Constant Gamma 3; G3m Marker; Immunoglobulin Gm3

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range3.12-200ng/mL
  • Applicable Sensitivity1.48ng/mL
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  • ELISA Kit DIY Materials for Immunoglobulin G3 (IgG3) Packages (Simulation)
  • ELISA Kit DIY Materials for Immunoglobulin G3 (IgG3) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Immunoglobulin G3 (IgG3) .No significant cross-reactivity or interference between Immunoglobulin G3 (IgG3) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingHindawi:Source
  • Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancerPubmed:25529861
  • The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingPubMed: 26161394

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