ELISA Kit DIY Materials for Interferon Gamma Induced Protein 10kDa (IP10) Homo sapiens (Human) Assay Kit DIY Materials

CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.6-1,000pg/mL
  • Applicable Sensitivity5.5pg/mL
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  • ELISA Kit DIY Materials for Interferon Gamma Induced Protein 10kDa (IP10) Packages (Simulation)
  • ELISA Kit DIY Materials for Interferon Gamma Induced Protein 10kDa (IP10) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Interferon Gamma Induced Protein 10kDa (IP10).No significant cross-reactivity or interference between Interferon Gamma Induced Protein 10kDa (IP10) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Melatonin inhibits type 1 interferon signaling of toll-like receptor 4 via heme oxygenase-1 induction in hepatic ischemia/reperfusion.PubMed: 22288937
  • Maternal circulating leukocytes display early chemotactic responsiveness during late gestationPubMed: PMC3561147
  • Serum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186
  • The Immunosuppressant Protosappanin A Diminished Recipient T Cell Migration into Allograft via Inhibition of IP-10 in Rat Heart TransplantPubmed:24798458
  • The G-Protein-Coupled Bile Acid Receptor Gpbar1 (TGR5) Inhibits Gastric Inflammation Through Antagonizing NF-κB Signaling PathwayPubMed: 26696888
  • Hypoxia/ischemia promotes CXCL10 expression in cardiac microvascular endothelial cells by NFkB activationPubmed:26891076
  • LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4.pubmed:28641579
  • Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumorsPubmed: 30952517
  • Integrated Omics Reveals Tollip as an Aggravator and Therapeutic Target for Hepatic Ischemia‐Reperfusion Injury in MicePubmed: 31077413
  • IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancerPubmed: 31173199
  • Inflammatory Responses of Astrocytes Are Independent from Lipocalin 2Pubmed: 32959226

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