ELISA Kit DIY Materials for Kininogen 1 (KNG1)
BDK; KNG; HMWK; Alpha-2-Thiol Proteinase Inhibitor; Bradykinin; Kallidin; Fitzgerald factor; High molecular weight kininogen; Williams-Fitzgerald-Flaujeac factor; Ile-Ser-Bradykinin
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSB267Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryHematology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleplasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.068ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Kininogen 1 (KNG1). No significant cross-reactivity or interference between Kininogen 1 (KNG1) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes AnalysisPubMed: 26098644
- Einfluss zyklischer mechanischer Dehnung auf das Kinin-Kallikrein-System in alveolären Typ-II-Zellen der Ratte21931
- Evaluation of the anti-hypertensive effect of Tengfu Jiangya tablet by combination of UPLC-Q-exactive-MS-based metabolomics and iTRAQ-based proteomics …Pubmed:29453042
- Contact System Activation and Neutrophil Extracellular Trap Markers: Risk Factors for Portal Vein Thrombosis in Patients With Hepatocellular CarcinomaDoi: 10.1177/1076029618825310
- Activation of Factor XII and Kallikrein-kinin System Combined with Neutrophil Extracellular Trap Formation in Diabetic RetinopathyPubmed: 31426112
- Einfluss mechanischer Dehnung von alveolaren Typ-II-Zellen auf die Bildung von Bradykinin durch das Kallikrein-Kinin-System
- Maternal Serum Proteomic Profiles of Pregnant Women With Type 1 Diabetes34940989