ELISA Kit DIY Materials for Laminin (LN) 
LAM
- UOM
- FOB US$ 1,037.00 US$ 1,728.00 US$ 3,110.00 US$ 6,048.00 US$ 10,368.00
- Quantity
Overview
Properties
- Product No.KSA082Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- Category
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.781-50ng/mL
- Applicable Sensitivity0.28ng/mL.
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Laminin (LN).No significant cross-reactivity or interference between Laminin (LN) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jellyPubmed: 24739658
- Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented mediaPubmed:Pmc4130592
- Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progressionPubmed:24807734
- Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis.Pubmed:24813083
- Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile HemangiomasPubMed: 26430624
- Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cellsDoi: Abs
- Clinical significance of serum laminin and typeâIV collagen levels in cutaneous melanoma patientsPubmed:27330797
- Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation.pubmed:27456745
- Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Responsepubmed:29172262
- Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in AdipocytesPubmed:29986925
- Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis10.1007:s00217-018-3119-z
- High amplitude stretching of ATII cells and fibroblasts results in profibrotic effectsPubmed: 31290711
- Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve RepairPubmed: 31758411
- Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellulärmatrix von Kalbs-Stimmlippen
- CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE