ELISA Kit DIY Materials for Low Density Lipoprotein (LDL) Homo sapiens (Human) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range7.81-500ng/mL
  • Applicable Sensitivity3.6ng/mL
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  • ELISA Kit DIY Materials for Low Density Lipoprotein (LDL) Packages (Simulation)
  • ELISA Kit DIY Materials for Low Density Lipoprotein (LDL) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Low Density Lipoprotein (LDL). No significant cross-reactivity or interference between Low Density Lipoprotein (LDL) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL.NCBI: PMC3896367
  • Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDLPubmed:24465540
  • PCSK9 deficiency unmasks a sex-and tissue-specific subcellular distribution of the LDL and VLDL receptors in micePubMed: 26323289
  • 27-Hydroxycholesterol contributes to disruptive effects on learning and memory by modulating cholesterol metabolism in the rat brainPubMed: 25987203
  • Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in micePubmed:27142749
  • Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese miceS1756464617300555
  • Combined Effects of Curcumin and Lycopene or Bixin in Yoghurt on Inhibition of LDL Oxidation and Increases in HDL and Paraoxonase Levels in Streptozotocin-Diabetic Rats.pubmed:28333071
  • THE FUNCTION OF SIALIC ACID AS A RADICAL SCAVENGER IN EXPERIMENTAL HYPOTHYROIDISM WITH AND WITHOUT HYPERLIPIDEMIA. Hiperlipidemili ve …163
  • Red paprika (Capsicum annuum L.) and its main carotenoid capsanthin ameliorate impaired lipid metabolism in the liver and adipose tissue of high-fat diet-induced obese mice 10.1016:j.jff.2017.01.044
  • Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients pubmed:28954218
  • Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 releasepubmed:29146189
  • Anthocyanin Cyanidin-3-Glucoside Attenuates Platelet Granule Release in Mice Fed High-Fat Diets.pubmed:28978870
  • Bifenthrin exerts proatherogenic effects via arterial accumulation of native and oxidized LDL in rats: the beneficial role of vitamin E and seleniumPubmed: 30465240
  • Alteration of circulating innate lymphoid cells in patients with atherosclerotic cerebral infarctionPubmed: 30662674
  • ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagyPubmed: 31358770
  • ¡°Adjusting Internal Organs and Dredging Channel¡± Electroacupuncture Ameliorates Insulin Resistance in Type 2 Diabetes Mellitus by Regulating the Intestinal?¡­34135611
  • Effect of Acupoint Embedding on Serum Leptin and Hypothalamus Leptin Receptor Expression in Rats with Simple Obesity34659432
  • High-fat diet-induced aggravation of cardiovascular impairment in permethrin-treated Wistar rats34224971
  • Brown and golden flaxseed reduce intestinal permeability and endotoxemia, and improve the lipid profile in perimenopausal overweight womenPubmed:35311432

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