ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a) Homo sapiens (Human) Assay Kit DIY Materials

CCL3; MIP1-A; SCYA3; Chemokine C-C-Motif Ligand 3; Small Inducible Cytokine A3; Homologous To Mouse Mip-1a; Tonsillar Lymphocyte LD78 Alpha Protein

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range15.62-1000pg/mL
  • Applicable Sensitivity5.5pg/mL.
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  • ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a) Packages (Simulation)
  • ELISA Kit DIY Materials for Macrophage Inflammatory Protein 1 Alpha (MIP1a) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Macrophage Inflammatory Protein 1 Alpha (MIP1a).No significant cross-reactivity or interference between Macrophage Inflammatory Protein 1 Alpha (MIP1a) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Age influence on hypersensitivity pneumonitis induced in mice by exposure to Pantoea agglomeransPubmed: 24044680
  • The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37RaPubmed:26912204
  • CIRCULATING MACROPHAGE INFLAMMATORY PROTEIN-1 ALPHA (MIP1-Α) AS A POTENTIAL BIOMARKER FOR THE DIAGNOSIS OF PATIENTS WITH …57e97ac908aeb34bc08fd41a.pdf
  • Role of Gut-Derived Endotoxin on Type I Collagen Production in the Rat Pancreas after Chronic Alcohol Exposure pubmed:29121396
  • Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without posttraumatic stress disorder10.1016/j.pharep.2017.08.008
  • Molybdenum disulfide/graphene oxide nanocomposites show favorable lung targeting and enhanced drug loading/tumor-killing efficacy with improved …Articles:am2017225
  • Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis RatPubmed:29682587
  • Cathelicidin LL-37 affects surface and intracellular Toll-like receptor expression in tissue mast cellsPubmed:29670923
  • Changes in the concentrations of inflammatory and oxidative status biomediators (MIP-1 α, PMN elastase, MDA, and IL-12) in depressed patients with and without …Pubmed:29339257
  • CQPC06 Activity Prevents Dextran Sulfate Sodium‐Induced Colitis by Regulating the IL‐8 PathwayDoi: 10.1111/1750-3841.14346
  • The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusionPubmed: 30558792
  • Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stressPubmed: 33227274
  • α-Galactosylceramide and its analog OCH differentially affect the pathogenesis of ISO-induced cardiac injury in micePubmed: 32973325
  • PIGU promotes hepatocellular carcinoma progression through activating NF-κB pathway and increasing immune escapePubmed: 32971102
  • A vaccine-based nanosystem for initiating innate immunity and improving tumor immunotherapyPubmed: 32332752
  • PDIA6 promotes pancreatic cancer progression and immune escape through CSN5-mediated deubiquitination of β-catenin and PD-L134325342

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