ELISA Kit DIY Materials for Mannose Binding Lectin (MBL)
MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect)
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSB480Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transductionInfection immunityImmune molecule
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.067ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Mannose Binding Lectin (MBL). No significant cross-reactivity or interference between Mannose Binding Lectin (MBL) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Analysis of the Urine Proteome of Human Contrast-Induced Kidney Injury Using Two-Dimensional Fluorescence Differential Gel Electrophoresis/Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry/Liquid Chromatography Mass Spectrometry. Karger: 000255439
- Serum Levels and H/L Gene Polymorphism of Mannose-Binding Lectin in Primary Open Angle GlaucomaIngenta: art00007
- Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease.PubMed: 22941511
- Urinary mannose-binding lectin is a biomarker for predicting the progression of immunoglobulin (Ig)A nephropathyPubMed: PMC3406374
- Alternative Complement Pathway Activation Products in Urine and Kidneys of Patients with ANCA-Associated GNPubmed: 24115193
- Novel findings on the role of ficolins and colectins in the innate response against Leishmania braziliensisPubmed: 32827456
- Complement activation profile of patients with primary focal segmental glomerulosclerosisPubmed: 32569286
- The lectin pathway of complement and the initial recognition of Leishmania infantum promastigotes34242658