ELISA Kit DIY Materials for Matrix Gla Protein (MGP)

MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,300.00 US$ 2,166.00 US$ 3,899.00 US$ 7,581.00 US$ 12,996.00
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Overview

Properties

Product No.KSB477Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryMetabolic pathway Hematology Bone metabolism

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.781-50ng/mL
Applicable Sensitivity0.28ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Matrix Gla Protein (MGP). No significant cross-reactivity or interference between Matrix Gla Protein (MGP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cellsPubMed: PMC3611891
Bone metabolism regulators and arterial stiffness in postmenopausal womenPubmed: 23916080
Osteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activityScienceDirect: S1110116414000076
Canola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in miceResearchgate:Source
Markers of increased cardiovascular risk in patients with chronic kidney diseaseBiomedcentral:Source
The behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veinsHvm:Source
Effect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5PubMed: 26176325
Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary studypmc:PMC4739105
Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney DiseasePubmed:27100101
Matrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculuspubmed:27348051
Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitisPubmed:29880866
High concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla proteinPubmed:29935264
Serum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseasesPubmed: 30597137
Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis PatientsPubmed: 30847765
Relationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patientsPubmed: 31538831
Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4Pubmed: 32322728