ELISA Kit DIY Materials for Mucin 2 (MUC2) Mus musculus (Mouse) Assay Kit DIY Materials

MLP; SMUC; Oligomeric Mucus/Gel-Forming; Mucin 2,Intestinal/Tracheal

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.78-50ng/mL
  • Applicable Sensitivity0.30ng/mL
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  • ELISA Kit DIY Materials for Mucin 2 (MUC2) Packages (Simulation)
  • ELISA Kit DIY Materials for Mucin 2 (MUC2) Packages (Simulation)
  • KSA705Mu01.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Mucin 2 (MUC2). No significant cross-reactivity or interference between Mucin 2 (MUC2) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.PubMed: 20717734
  • Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats.Pubmed:24627600
  • Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosisPubMed: 26217323
  • Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathwayPubMed: 26244071
  • Secreted mucins and airway bacterial colonization in non-CF bronchiectasisPubMed: 26172851
  • Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonizationdoi:10.1513
  • An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
  • Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA10.21769:BioProtoc.2879
  • The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung diseasePubmed: 30573494
  • Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast CancerPubmed: 30682816
  • PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific MetabolismPubmed: 31564549
  • Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic RatsPubmed: 32144600
  • Mucus layer modeling of human colonoids during infection with enteroaggragative E. coliPubmed: 32601325
  • Defective intestinal repair after short-term high fat diet due to loss of efferocytosis
  • Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis34145945
  • Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced …34370080

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