ELISA Kit DIY Materials for Myelin Basic Protein (MBP)
Myelin A1 protein; Myelin membrane encephalitogenic protein
- UOM
- FOB US$ 1,134.00 US$ 1,890.00 US$ 3,402.00 US$ 6,615.00 US$ 11,340.00
- Quantity
Overview
Properties
- Product No.KSA539Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryInfection immunityNeuro scienceAutoimmunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cerebrospinal fluid, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.055ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Myelin Basic Protein (MBP). No significant cross-reactivity or interference between Myelin Basic Protein (MBP) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- CD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
- Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat BrainPubmed: 23183024
- Myelin basic protein and ischemia modified albumin levels in acute ischemic stroke casesPubMed: 26648996
- Morphology and staining behavior of neutrophilic and eosinophilic granulocytes of the common marmoset (Callithrix jacchus)Pubmed:27165445
- Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
- Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord.pubmed:27573615
- Glial markers and emotional memory in rats following acute cerebral radiofrequency exposures.pubmed:27696165
- Serum microtubule associated protein tau and myelin basic protein as the potential markers of brain ischaemia-reperfusion injury in patients undergoing carotid endarterectomyAA.2016.0008
- Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosispubmed:28489854
- Acrolein-mediated neurotoxicity in growing Wistar male ratsPubmed:30033014
- Association and Risk of Canine Distemper with Respect to Age, Sex and Breed of Dogs Suffering from Demyelinating Neuropathies10.5455:ijlr.20170811080455
- Biosensors and nanobiosensors for rapid detection of autoimmune diseases: a reviewPubmed: 31760523
- The role of light desynchronosis in the development of stress-induced aging
- Association of eosinophil-mediated inflammatory biomarkers with the presence of the Schatzki ring34091432
- Searching for Noninvasive Predictors of the Diagnosis and Monitoring of Eosinophilic Esophagitis¡ªThe Importance of Biomarkers of the Inflammatory Reaction?¡
- Monitoring autoimmune diseases by bioelectrochemical detection of autoantibodies. Application to the determination of anti-myelin basic protein autoantibodies in …Pubmed:35202838