ELISA Kit DIY Materials for Myoglobin (MYO)
PVALB; MB
- UOM
- FOB US$ 716.00 US$ 1,194.00 US$ 2,149.00 US$ 4,179.00 US$ 7,164.00
- Quantity
Overview
Properties
- Product No.KSA480Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryCardiovascular biology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.054ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Myoglobin (MYO).No significant cross-reactivity or interference between Myoglobin (MYO) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Acute leukocyte, cytokine and adipocytokine responses to maximal and hypertrophic resistance exercise boutsPubmed:25145982
- Transcriptional Alterations by Ischaemic Postconditioning in a Pig Infarction Model: Impact on Microvascular ProtectionPubmed: 30650650
- Electrochemical dual-aptamer-based biosensor for nonenzymatic detection of cardiac troponin I by nanohybrid electrocatalysts labeling combined with DNA …Pubmed: 30954926
- DNA nanotetrahedron linked dual-aptamer based voltammetric aptasensor for cardiac troponin I using a magnetic metal-organic framework as a labelPubmed: 31123904
- Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions34914377
- A rapid fluorescent aptasensor for point-of-care detection of C-reactive proteinPubmed:35714415