ELISA Kit DIY Materials for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Homo sapiens (Human) Assay Kit DIY Materials

NT-Pro-BNP; ; N-BNP

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range39-2500pg/mL
  • Applicable Sensitivity15pg/mL
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  • ELISA Kit DIY Materials for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Packages (Simulation)
  • ELISA Kit DIY Materials for N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP).No significant cross-reactivity or interference between N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • The role of serum n-terminal pro-brain natriuretic peptide in transient tachypnea of the newbornEuropeanreview: Source
  • Vascular Adhesion Protein-1, a Novel Molecule, in Kidney and Heart Allograft RecipientsPubmed: 23769096
  • Paradoxical low-flow, low-gradient aortic stenosis despite preserved left ventricular ejection fraction: new insights from weights of operatively excised aortic valvesPubmed: 24755006
  • A Randomized Controlled Trial to Study the Effect of Yoga Therapy on Cardiac Function and N Terminal Pro BNP in Heart FailureNCBI: PMC3981569
  • Diabetes enhances the intrahepatic vascular response to endothelin-1 in cirrhotic rats: association with the ETA receptor and pERK up-regulation.Pubmed:24636620
  • Growth-differentiation factor 15 and osteoprotegerin in acute myocardial infarction complicated by cardiogenic shock: a biomarker substudy of the IABP-SHOCK II-trial.Pubmed:24903195
  • Biomarkers and Echocardiographic Predictors of Myocardial Dysfunction in Patients with HypertensionPubMed: 25747153
  • The Multi-Biomarker Approach for Heart Failure in Patients with HypertensionPubMed: 25984599
  • Effect of Treatment on Body Fluid in Patients with Unilateral Aldosterone Producing Adenoma: Adrenalectomy versus SpironolactonePubMed: 26477337
  • Diabetes enhances the intrahepatic vascular response to endothelin‐1 in cirrhotic rats: association with the ETA receptor and pERK up‐regulationPubMed: 24636620
  • Qiliqiangxin improves cardiac function and attenuates cardiac remodeling in rats with experimental myocardial infarctionPubMed: 26261541
  • NT-proBNP in Children With Left to Right Shunt and Dilated Cardiomyopathyserve:4485
  • Human myoblast transplantation in mice infarcted heart alters the expression profile of cardiac genes associated with left ventricle remodelingPubmed:26457413
  • Cardiovascular changes in patients with non-severe Plasmodium vivax malariaarticle:S2352-9067(16)30010-0
  • Soluble Glycoprotein 130 and Heat Shock Protein 27 as Novel Candidate Biomarkers of Chronic Heart Failure with Preserved Ejection FractionPubmed:27067668
  • Differences in biochemical and genetic biomarkers in patients with heart failure of various etiologies.pubmed:27448535
  • Expression of pericardial fluid T-cells and related inflammatory cytokines in patients with chronic heart failure.pubmed:28565777
  • Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure.pubmed:28127817
  • Study of Vitamin D Status in Patients with Dilated Cardiomyopathy at a Teaching Hospital in North India.pubmed:28465969
  • 5-methoxytryptophan is a potential marker for post-myocardial infarction heart failure - apreliminary approach to clinical utility.pubmed:27526355
  • Beneficial Effects of Ozone Therapy on Oxidative Stress, Cardiac Functions and Clinical Findings in Patients with Heart Failure Reduced Ejection Fractionpubmed:28097518
  • Serial heart rhythm complexity changes in patients with anterior wall ST segment elevation myocardial infarction.pubmed:28252107
  • The predictive capabilities of a novel cardiovascular magnetic resonance derived marker of cardiopulmonary reserve on established prognostic surrogate markers in patients with pulmonary vascular disease: results of a longitudinal pilot studypubmed:28065166
  • AVALIAÇÃO ELETROCARDIOGRÁFICA AMBULATORIAL DE CÃES COM EHRLIQUIOSE MONOCÍTICA CRÔNICAhandle:11449
  • Cardiac biomarkers in dogs with visceral leishmaniasisS0301-732X2016000300004
  • Evaluation of N-Terminal Pro Brain Natriuretic Peptide as a biomarker for clinical severity of heart failure in pediatric populationAttach432
  • Cardioprotection against Heart Failure by Shenfu Injection via TGF-β/Smads Signaling Pathwaypubmed:28698735
  • QSKL protects against myocardial apoptosis on heart failure via PI3K/Akt-p53 signaling pathway.pubmed:29209026
  • Reversal effects of low-dose imatinib compared with sunitinib on monocrotaline-induced pulmonary and right ventricular remodeling in ratspubmed:29100963
  • Qiliqiangxin Enhances Cardiac Glucose Metabolism and Improves Diastolic Function in Spontaneously Hypertensive Ratspubmed:28706558
  • Prediction of electro-anatomical substrate using APPLE score and biomarkersPubmed:29893827
  • Role of NT-proANP and NT-proBNP in patients with atrial fibrillation: Association with atrial fibrillation progression phenotypesPubmed:29604419
  • MicroRNAs in Peripheral Mononuclear Cells as Potential Biomarkers in Hypertensive Patients With Heart Failure With Preserved Ejection FractionPubmed:29506053
  • Differential microRNA expression profiles and bioinformatics analysis between young and aging spontaneously hypertensive ratsPubmed:29328372
  • Regulation of circulating chromogranin B levels in heart failurePubmed:29098879
  • Effect of 6-min Walk Test on pro-BNP Levels in Patients with Pulmonary Arterial HypertensionPubmed:29564533
  • Dynamic monitoring the level of NT-proBNP in assessing the value of cardiac function and prognosis in patients with systemic infectionijcem0072283.pdf
  • Signature-oriented investigation of the efficacy of multicomponent drugs against heart failurePubmed: 30230922
  • Qiliqiangxin ameliorates cardiac dysfunction in heart failure rats post myocardial infarction via activation of HIF/VEGF pathway
  • Decreasing cardiac iron overload with Amlodipine and Spirulina in children with β-thalassemiaDoi: 10.1016/j.phoj.2018.10.001
  • Acute Responses of Novel Cardiac Biomarkers to a 24-h Ultra-MarathonPubmed: 30625976
  • Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure.
  • Biological markers for the effects of yoga as a complementary and alternative medicinePubmed: 30735481
  • Trimetazidine Attenuates Exhaustive Exercise-Induced Myocardial Injury in Rats via Regulation of the Nrf2/NF-κB Signaling PathwayPubmed: 30890937
  • Potential Effects of CXCL9 and CCL20 on Cardiac Fibrosis in Patients with Myocardial Infarction and Isoproterenol-Treated RatsPubmed: 31083544
  • Evaluation of heart rate variability and behavior of electrocardiographic parameters in dogs affected by chronic Monocytic EhrlichiosisPubmed: 31125348
  • Cardio-protective effect of the secretome of Sca-1+ and Sca-1-cells in heart failure–not equal, but equally important? Paracrine cell effects on heart failure
  • The potential role of inflammation in cryptogenic stroke
  • Arterial Stiffness and Indices of Left Ventricular Diastolic Dysfunction in Patients with Embolic Stroke of Undetermined EtiologyPubmed: 31612072
  • Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle HypertrophyPubmed: 32148580
  • Preclinical development of a miR-132 inhibitor for heart failure treatmentPubmed: 32005803
  • Evaluating the Relationship of GDF-15 with Clinical Characteristics, Cardinal Features, and Survival in Multiple MyelomaPubmed: 33144847
  • CDR132L improves systolic and diastolic function in a large animal model of chronic heart failurePubmed: 33089304
  • Omega-3 polyunsaturated fatty acids alleviate hydrogen sulfide-induced blood-testis barrier disruption in the testes of adult micePubmed: 33068716
  • Apela improves cardiac and renal function in mice with acute myocardial infarctionPubmed: 32686917
  • Left Ventricular Diastolic Dysfunction as Predictor of Unfavorable Prognosis After ESUS33727824
  • κ Opioid Receptor Agonist Inhibits Myocardial Injury in Heart Failure Rats through Activating Nrf2/HO-1 Pathway and Regulating Ca2+-SERCA2a34373768
  • Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy34356497
  • Risk Stratification of Pulmonary Thromboembolism using Brain Natriuretic Peptide and Troponin I; a Brief ReportPubmed:35072097
  • Interplay between mineral bone disorder and cardiac damage in acute kidney injury: from Ca2+ mishandling and preventive role of Klotho in mice to its potential …Pubmed:35077866
  • The Key Role of Hepcidin-25 in Anemia in Multiple Myeloma Patients with Renal ImpairmentPubmed:35334593
  • Prothymosin α Gene Transfer Modulates Myocardial Remodeling after Ischemia-Reperfusion InjuryPubmed:35273440

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