ELISA Kit DIY Materials for Neuropilin 1 (NRP1) Homo sapiens (Human) Assay Kit DIY Materials

CD304; BDCA4; VEGF165R; Vascular endothelial cell growth factor 165 receptor

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range62.5-4000pg/mL
  • Applicable Sensitivity26.2pg/mL
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  • ELISA Kit DIY Materials for Neuropilin 1 (NRP1) Packages (Simulation)
  • ELISA Kit DIY Materials for Neuropilin 1 (NRP1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Neuropilin 1 (NRP1). No significant cross-reactivity or interference between Neuropilin 1 (NRP1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Peritumoral brain edema in angiomatous supratentorial meningiomas: an investigation of the vascular endothelial growth factor A pathwayPubMed: 23398358
  • Circulating Soluble Neuropilin-1 in Patients with Early Cervical Cancer and Cervical Intraepithelial Neoplasia Can Be Used as a Valuable Diagnostic BiomarkerPubmed:25873749
  • Cytokine-CpG Motif Oligodeoxynucleotide Co-inoculation in the Murine Malaria Modelhandle:123456789
  • Reduced Sympathetic Innervation in Endometriosis is Associated to Semaphorin 3C and 3FExpression.pubmed:27558236
  • Serum concentrations of neuropilin-1 in women with endometriosisPubmed: 32069143
  • Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway.Pubmed: 32603817
  • SARS-CoV2 Induced Biochemical Mechanisms in Liver Damage and Intestinal Lesions.

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