ELISA Kit DIY Materials for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)
NAP3; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein
- UOM
- FOB US$ 842.00 US$ 1,404.00 US$ 2,527.00 US$ 4,914.00 US$ 8,424.00
- Quantity
Overview
Properties
- Product No.KSA041Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryCytokineTumor immunityInfection immunity
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.6-1,000pg/mL
- Applicable Sensitivity6.1pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Neutrophil Activating Protein 3 (NAP3).No significant cross-reactivity or interference between Neutrophil Activating Protein 3 (NAP3) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- 白细胞介素10 上调表达对家兔慢性细菌性鼻窦炎黏膜创伤修复的影响article:11593
- Epidermal CD147 expression plays a key role in IL-22-induced psoriatic dermatitis.pubmed:28272440
- Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke‐induced airway inflammationPubmed: 31209984
- CXCL1 as an Unfavorable Prognosis Factor Negatively Regulated by DACH1 in Non-small Cell Lung CancerPubmed: 31998654
- Baohuoside I suppresses breast cancer metastasis by downregulating the tumor-associated macrophages/CXC motif chemokine ligand 1 pathwayPubmed: 32911383
- Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitisPubmed: 32709888
- Aiduqing formula suppresses breast cancer metastasis via inhibiting CXCL1-mediated autophagy
- Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration34461944
- Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice34960627