ELISA Kit DIY Materials for Neutrophil gelatinase-associated lipocalin (NGAL) Canis familiaris; Canine (Dog) Assay Kit DIY Materials

Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range9.4-600ng/mL
  • Applicable Sensitivity3.5ng/mL
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  • ELISA Kit DIY Materials for Neutrophil gelatinase-associated lipocalin (NGAL) Packages (Simulation)
  • ELISA Kit DIY Materials for Neutrophil gelatinase-associated lipocalin (NGAL) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Neutrophil Gelatinase Associated Lipocalin (NGAL). No significant cross-reactivity or interference between Neutrophil Gelatinase Associated Lipocalin (NGAL) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancyPubMed: PMC3794962
  • Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 PathwayPubmed: 24369464
  • Lipocalin-2: Response To Resistance Training in Obese Young MenIjssjournal: Source
  • Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young MenTums: Source
  • Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlapPubmed: 23794464
  • Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathyPubmed:24802166
  • Nephrotoxic effects of varenicline as the most effective drug used for smoking cessation: a preliminary experimental studyPubmed:25812822
  • 血清及胆汁中NGAL 对于区分良恶性胆道狭窄的临床意义Wjgnet:Source
  • Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty ratsSagepub:Source
  • Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese MenBiomedpharmajournal:Source
  • A Study On Thyroid Hormone And Its Effects On Exercise Ifcss:Source
  • 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义Jcp-Sh:Source
  • Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancyPubmed:Pmc4260273
  • Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary diseasePubmed:Pmc4043430
  • Impact of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal modelPubmed:24916275
  • Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous CrisisPubmed:25448261
  • Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitisPubMed: 26191125
  • Increased cardiac index attenuates septic acute kidney injury: a prospective observational studyPubMed: 25745357
  • 中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义Journal: Fulltext
  • Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in RatsPubMed: 25909514
  • Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal modelPubmed:26714833
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.Pubmed:26991748
  • Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu
  • The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in ratspubmed:27430552
  • Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis.pubmed:27775820
  • Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN)pubmed:28659153
  • From varices to venous ulceration: the story of chronic venous disease described by metalloproteinasespubmed:26991748
  • Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.pubmed:29027259
  • Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac SurgeryPubmed:29386545
  • Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activityPubmed:30036488
  • Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic NephropathyPubmed:29642511
  • Improved approach for normothermic machine perfusion of cold stored kidney graftsPubmed:30018731
  • Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypesISBN:978-951-51-4039-5
  • Potential Novel Biomarkers of Obstructive Nephropathy in Children with HydronephrosisDoi: 10.1155/2018/1015726
  • The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic NephropathyPubmed: 30504713
  • Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicinePubmed: 30871515
  • Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascadePubmed: 31197431
  • A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activationPubmed: 31447572
  • Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixturesPubmed: 31639605
  • Alkaline phosphatase treatment of Acute Kidney injury in an infant piglet Model of cardiopulmonary Bypass with Deep Hypothermic circulatory ArrestPubmed: 31578351
  • The evaluation of acute kidney injury due to ischemia by urinary neutrophil gelatinase-induced lipocalin (uNGAL) measurement in patients who underwent partial …Pubmed: 32980930
  • Upregulated miR-101 inhibits acute kidney injury–chronic kidney disease transition by regulating epithelial–mesenchymal transitionPubmed: 32633566
  • CОСТОЯНИЕ ТИРЕОИДНОЙ ФУНКЦИИ У ПАЦИЕНТОВ С САХАРНЫМ ДИАБЕТОМ 1-ГО ТИПА И ХРОНИЧЕСКОЙ БОЛЕЗНЬЮ ПОЧЕК …
  • Thyroid function in patients with type 1 diabetes mellitus and chronic kidney disease receiving renal replacement therapy
  • Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers33423326
  • Nrf2 deficiency aggravates the kidney injury induced by subacute cadmium exposure in mice33398418
  • Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury33604384
  • Elevated plasma level of neutrophil gelatinase-associated lipocalin (NGAL) in patients with breast cancer34104101
  • Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental …34169407
  • Preservation of Mitochondrial Coupling and Renal Function by Controlled Oxygenated Rewarming of Porcine Kidney Grafts34944524
  • SERS based Y-shaped aptasensor for early diagnosis of acute kidney injuryPubmed:35733690

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