ELISA Kit DIY Materials for Osteonectin (ON)
SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSA791Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryMetabolic pathwayTumor immunityInfection immunityHepatologyBone metabolism
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.625-40ng/mL
- Applicable Sensitivity0.242ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Osteonectin (ON).No significant cross-reactivity or interference between Osteonectin (ON) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistancePubMed: 26199111
- Effect of resistance ladder training on SPARC expression in skeletal muscle of hindlimb immobilized ratsPubMed: 26467437
- An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic CancerPubMed: 26266370
- A proteomics-based approach identifies secreted protein acidic and rich in cysteine as a prognostic biomarker in malignant pleural mesotheliomaPubmed:26889976
- Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria.pubmed:27916484
- Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellituspubmed:29078839
- Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancerDoi: 10.1177/2057178X18800534
- Osteoconductive potential of PLGA/bioglass composite biomaterials