ELISA Kit DIY Materials for Osteopontin (OPN) Mus musculus (Mouse) Assay Kit DIY Materials

SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range31.2-2000pg/mL
  • Applicable Sensitivity14.2pg/mL
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  • ELISA Kit DIY Materials for Osteopontin (OPN) Packages (Simulation)
  • ELISA Kit DIY Materials for Osteopontin (OPN) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Osteopontin (OPN).No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culturePubMed: 19280635
  • Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patientsPubMed: 19747583
  • Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis.Jrheum: source
  • Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester)ScienceDirect: S0378517312003742
  • The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineeringEcmjournal: Source
  • Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian HorseScienceDirect: Source
  • Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory ConditionsWiley:Source
  • Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in OsteoblastsPubmed:24905552
  • Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of ratsPubmed:25038619
  • The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disordersPubmed:25245337
  • Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cellsPubmed:25542352
  • Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesisPubMed: 26283150
  • An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic CancerPubMed: 26266370
  • Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based HydrogelContent: Asp
  • Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancerIndex.Php: Amor
  • Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 PathwayPubMed: 26327817
  • Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma.Ebscohost
  • Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.Pubmed:26963343
  • Stenotic Bicuspid and Tricuspid Aortic Valves　- Micro-Computed Tomography and Biological Indices of Calcification.pubmed:28344201
  • Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure.pubmed:28127817
  • Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes.pubmed:28337262
  • Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humanspubmed:29212824
  • Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation.pubmed:28742995
  • Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental MethodsPubmed:30040720
  • Osteopontin, cardiovascular risk factors and carotid intima-Media thickness in chronic kidney diseaseid:233806
  • In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem CellsDoi: 10.1016/j.carbon.2018.10.081
  • Detection of proteins associated with extracellular matrix regulation in the aqueous humour of patients with primary glaucoma.Pubmed: 30994369
  • Identification of urinary candidate biomarkers of cisplatin-induced nephrotoxicity in patients with carcinomaPubmed: 31629959
  • Inflammatory response to magnesium-based biodegradable implant materialsPubmed: 31610341
  • Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and …Pubmed: 32182704
  • Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in ratsPubmed: 33010420
  • Osteoconductive potential of PLGA/bioglass composite biomaterials
  • Structural Constraint of Osteopontin Facilitates Efficient Binding to CD4434070790
  • Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells
  • Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate …34857049
  • Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot StudyPubmed:35743980

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