ELISA Kit DIY Materials for Peroxisome Proliferator Activated Receptor Gamma (PPARg) Rattus norvegicus (Rat) Assay Kit DIY Materials

PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids。
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.056ng/mL
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  • ELISA Kit DIY Materials for Peroxisome Proliferator Activated Receptor Gamma (PPARg) Packages (Simulation)
  • ELISA Kit DIY Materials for Peroxisome Proliferator Activated Receptor Gamma (PPARg) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Gamma (PPARg). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Gamma (PPARg) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Elevated levels of PPAR-gamma in the cerebrospinal fluid of patients with multiple sclerosisPubmed: 24021801
  • Nonivamide enhances miRNA let‐7d expression and decreases adipogenesis PPARγ expression in 3T3‐L1 cellsPubmed:25704235
  • Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazonePubMed: 26321377
  • Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantiapmc:PMC4866090
  • Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic RatsSupplemented with Momordica charantia.pubmed:27190792
  • Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone.pubmed:26321377
  • Unlike PPARgamma, neither other PPARs nor PGC-1alpha is elevated in the cerebrospinal fluid of patients with multiple sclerosispubmed:28483651
  • Engulfment of Hb‐activated platelets differentiates monocytes into pro‐inflammatory macrophages in PNH patientsPubmed:29677388
  • Simpson–Golabi–Behmel syndrome human adipocytes reveal a changing phenotype throughout differentiationPubmed:29574488
  • Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsiaPubmed: 31420792
  • Hyperglycemia Changes Expression of Key Adipogenesis Markers (C/EBPα and PPARᵞ) and Morphology of Differentiating Human Visceral AdipocytesPubmed: 31398873
  • Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cellsPubmed: 31916655
  • In©\vitro effect of pine bark extract on melanin synthesis, tyrosinase activity, production of endothelin©\1 and PPAR in cultured melanocytes exposed to Ultraviolet?¡­33960120
  • TRPA1 Agonist Cinnamaldehyde Decreases Adipogenesis in 3T3-L1 Cells More Potently than the Non-agonist Structural Analog Cinnamyl Isobutyrate33403292
  • Quantitative real-time measurement of endothelin-1-induced contraction in single non-activated hepatic stellate cells34343209
  • Maternal Vitamin D Deficiency Reduces Docosahexaenoic Acid, Placental Growth Factor and Peroxisome Proliferator Activated Receptor Gamma levels in the Pup …34768025
  • Melatonin attenuates cisplatin-induced acute kidney injury in mice: Involvement of PPARα and fatty acid oxidationPubmed:35367536

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