ELISA Kit DIY Materials for Platelet Factor 4 (PF4) Homo sapiens (Human) Assay Kit DIY Materials

CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, platelet-poor plasma and cell culture supernates.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100pg/mL
  • Applicable Sensitivity0.65pg/mL
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  • ELISA Kit DIY Materials for Platelet Factor 4 (PF4) Packages (Simulation)
  • ELISA Kit DIY Materials for Platelet Factor 4 (PF4) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Platelet Factor 4 (PF4).No significant cross-reactivity or interference between Platelet Factor 4 (PF4) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Propofol lowers serum PF4 level and partially corrects hypercoagulopathy in endotoxemic ratsPubMed: 20601223
  • Secretome of apoptotic peripheral blood cells (APOSEC) attenuates microvascular obstruction in a porcine closed chest reperfused acute myocardial infarction model: role of platelet aggregation and vasodilationPubMed: 22899170
  • Platelets Recognize Brain-Specific Glycolipid Structures, Respond to Neurovascular Damage and Promote NeuroinflammationPubMed: PMC3608633
  • Effect of splenectomy on platelet activation and decompression sickness outcome in a rat model of decompressionPubmed:25311322
  • A Phellinus baumii–based supplement containing Salvia miltiorrhiza Bunge improves atherothrombotic profiles through endothelial nitric oxide synthase and cyclooxygenase pathwaysin vitro and in vivoscience:S1756464616300676
  • Aspirin, but Not Tirofiban Displays Protective Effects in Endotoxin Induced Lung Injurypubmed:27583400
  • Evidence of the Role of R-Spondin 1 and Its Receptor Lgr4 in the Transmission of Mechanical Stimuli to Biological Signals for Bone Formation.pubmed:28272338
  • Feasibility of improving platelet‑rich plasma therapy by using chitosan with high platelet activation abilitypubmed:28450960
  • Aspirin pre-treatment modulates ozone-induced fetal growth restriction and alterations in uterine blood flow in ratsPubmed: 30528429
  • Short and long-term changes in platelet and inflammatory biomarkers after cryoballoon and radiofrequency ablationPubmed: 30857843
  • Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers
  • Newly Identified HNP‐F from Human Neutrophil Peptide 1 Promotes HemostasisPubmed: 30927490
  • Data for short and long-term prothrombotic biomarkers after cryoballoon and radiofrequency ablation
  • Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular MarkersPubmed: 32812629
  • The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in RodentsPubmed: 31533230
  • Feasibility study of use of rabbit blood to evaluate platelet activation by medical devicesPubmed: 31838449
  • The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets34099640
  • The effect of ChAdOx1 nCov-19 vaccine on arterial thrombosis development and platelet aggregation in female ratsPubmed:35183388

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