ELISA Kit DIY Materials for Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
CD254; TNFSF11; ODF; OPGL; TRANCE; HRANKL2; SOdf; Tumor Necrosis Factor(ligand)superfamily Member 11; TNF-related activation-induced cytokine
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSA855Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategorySignal transductionMetabolic pathwayApoptosisTumor immunityBone metabolism
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range15.6-1000pg/mL
- Applicable Sensitivity5.7pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). No significant cross-reactivity or interference between Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Compressive stress induced the up-regulation of M-CSF, RANKL, TNF-α expression and the down-regulation of OPG expression in PDL cells via the integrin-FAK pathwayScienceDirect: S0003996912003998
- Adipokines in Psoriatic Arthritis Patients: The Correlations with Osteoclast Precursors and Bone ErosionsPubMed: PMC3483160
- Regulatory effect of calcineurin inhibitor, tacrolimus, on IL-6/sIL-6R-mediated RANKL expression through JAK2-STAT3-SOCS3 signaling pathway in fibroblast-like synoviocytesPubMed: PMC3672788
- The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal modelPubmed: 23522850
- Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cellsPubmed: 24687841
- Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7Onlinelibrary: fsn3.109
- Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7Pubmed:Pmc4221832
- Effect of Nicotine on RANKL and OPG and Bone Mineral DensityPubmed:24830656
- Effects of icariin on the regulation of the OPG?RANKL?RANK system are mediated through the MAPK pathways in IL?1β?stimulated human SW1353 chondrosarcoma …Pubmed:25270538
- Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in ratsPubmed:25424914
- A novel model of bisphosphonate-related osteonecrosis of the jaw in ratsPubMed: 26191212
- MicroRNA-106b inhibits osteoclastogenesis and osteolysis by targeting RANKL in giant cell tumor of bonePubMed: 26053181
- Serum osteopontin levels are upregulated and predict disability after an ischaemic strokePubMed: 25845543
- Effetti del laser a bassa dose su cellule osteoblasticheINCERTIPARENTI_SERENA_TESI.pdf
- Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats.pubmed:27908549
- Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats.pubmed:28158228
- The effect of lamotrigine and phenytoin on bone turnover and bone strength: A prospective study in Wistar ratspubmed:27838501
- Odontoclastogenesis of mouse papilla-derived MDPC-23 cells induced by lipopolysaccharide.pubmed:28333374
- Sprint Interval Training Induces A Sexual Dimorphism but does not Improve Peak Bone Mass in Young and Healthy Mice.pubmed:28303909
- Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity.pubmed:28139685
- Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized ratsS0753332216322752
- SAT0003 The Role of 12/15-Lipoxygenase in The Pathogenesis of Osteoporosis in Micecontent:75
- Allogeneic yet major histocompatibility complex‑matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced trabecular bonearticle:10.1007
- Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopauseol.2017.6596
- Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in ratsPubmed:29574908
- Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats10.1016:j.jff.2018.04.048
- Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experimentPubmed:30015235
- The effect of levetiracetam on rat bone mineral density, bone structure and biochemical markers of bone metabolismPubmed:29428468
- Allogeneic yet major histocompatibility complex-matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced …Pubmed:28766138
- Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesisPubmed:29805533
- The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino ratsPubmed: 30208342
- Improved RANKL expression and osteoclastogenesis induction of CD27+ CD38− memory B cells: A link between B cells and alveolar bone damage in periodontitisPubmed: 30346027
- NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblastsPubmed: 30455992
- Alendronate Effect in RANKL Concentration at Ovariectomized Sprague Dawley Rat.
- Development and characterization of supramolecular calcitonin assembly and assessment of its interactions with the bone remodelling processPubmed: 30797058
- Evaluation of therapeutic effects of teriparatide in a rat model of zoledronic acid-induced bisphosphonate-related osteonecrosis
- Comparison of RANKL expression, inflammatory markers, and cardiovascular risk in patients with acute coronary syndrome with and without rheumatoid arthritisPubmed: 31297563
- Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritisPubmed: 31641939
- Mice lacking plastin-3 display a specific defect of cortical bone acquisitionPubmed: 31678489
- IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathwayPubmed: 32347300
- Anti-osteoporosis effect of Semen Cuscutae in ovariectomized mice through inhibition of bone resorption by osteoclasts34801609
- Inducible T‐cell co‐stimulator (ICOS) and ICOS ligand are novel players in the multiple‐myeloma microenvironmentPubmed:34954822
- Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axisPubmed:35453007
- Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK …Pubmed:35660352
- Identification of marker proteins in preterm and term birth: Relationship of rankl and HSP70 in the cord blood