ELISA Kit DIY Materials for Serum Amyloid A (SAA) Mus musculus (Mouse) Assay Kit DIY Materials

SA-A; SAA1; PIG4; TP53I4; Amyloid protein A; Amyloid fibril protein AA; Serum amyloid A-1 protein

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.62ng/mL
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  • ELISA Kit DIY Materials for Serum Amyloid A (SAA) Packages (Simulation)
  • ELISA Kit DIY Materials for Serum Amyloid A (SAA) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Serum Amyloid A (SAA).No significant cross-reactivity or interference between Serum Amyloid A (SAA) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effect of feeding a high fat diet on hydrogen sulfide (H 2 S) metabolism in the mousePubmed:24637018
  • Acute Phase Response: Implication in ST-segment Elevation Myocardial InfarctionPubmed:24894970
  • Comparison of the Effects of Enoxaparin and Heparin on Inflammatory Biomarkers in Patients with ST-segment Elevated Myocardial Infarction: A prospective Open Label Pilot Clinical TrialPubmed:25237354
  • No association between vitamin D levels and inflammation markers in patients with acute coronary syndromePubmed:25615956
  • Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical studyPubmed:25652121
  • EFFECT OF UTERINE IMMUNOMODULATION ON SERUM AMYLOID-A CONCENTRATION AND CONCEPTION RATE IN CYCLIC NON BREEDING COWSTcrjournals:Source
  • Impaired adipogenesis in adipose tissue associated with hepatic lipid deposition induced by chronic inflammation in mice with chew dietPubMed: 26188592
  • Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitusPubMed: 26049370
  • The effect of serum and follicular fluid amyloid-associated protein levels on in vitro fertilization outcome in patients with polycystic ovary syndromePubMed: 26463878
  • Circulating serum amyloid A: a potential biomarker for infection after aneurysmal subarachnoid hemorrhagefiles:ijcem0021201
  • An Anti-β-Amyloid Vaccine for Treating Cognitive Deficits in a Mouse Model of Down SyndromePubmed:27023444
  • PATHWAY SPECIFIC MARKERS FOR DIAGNOSING IRRITABLE BOWEL SYNDROMEy2016:0139148.html
  • Acute phase response in lame crossbred dairy cattlepubmed:27956769
  • A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defensePubmed:29410422
  • Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rationsPubmed:30060086
  • Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood …Pubmed: 30184125
  • Metabolism and immune status during transition period influences the lactation performance in Zebu (Bos indicus () cowsPubmed: 17430930
  • The predictive value of joint detection of serum amyloid protein A, PCT, and Hs-CRP in the diagnosis and efficacy of neonatal septicemia.Pubmed: 31298341
  • Evaluation of infrared thermography compared to rectal temperature to identify illness in early postpartum dairy cows
  • Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steersPubmed: 31410465
  • Effects of vaccination on acute-phase protein response in broiler chickenPubmed: 32191767
  • AA Amyloid Deposition in the Central and Peripheral Nervous Systems in FlamingosPubmed: 32677873
  • Serum amyloid A as an marker of cow֨ s mastitis caused by Streptococcus sp.Pubmed: 32505957
  • Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy …
  • РОЛЬ СЫВОРОТОЧНОГО АМИЛОИДА А И С-РЕАКТИВНОГО БЕЛКА В ПРОГНОЗИРОВАНИИ ПОСЛЕОПЕРАЦИОННЫХ ОСЛОЖНЕНИЙ И …
  • Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient?¡­33474564
  • Induction of Serum Amyloid A3 in Mouse Mammary Epithelial Cells Stimulated with Lipopolysaccharide and Lipoteichoic Acid34070499
  • Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats33510972
  • CCS e amiloide A do leite de quartos mam¨¢rios tratados na secagem com plasma rico em plaquetas aut¨®logo, associado ou n?o a antibi¨®tico
  • The Effect of Feed Supplementation with EM Bokashi® Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators …34596883
  • Effects of dietary turmeric (Curcuma longa) on innate and acquired immune responses in broiler chicken34765801
  • Mahanimbine Improved Aging-Related Memory Deficits in Mice through Enhanced Cholinergic Transmission and Suppressed Oxidative Stress, Amyloid Levels, and …Pubmed:35053756
  • A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPubmed:35329889
  • Serum and salivary adipokines in type 2 diabetes–Results of a pilot study in IndiaPubmed:35717896
  • Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patientsPubmed:35348620
  • İneklerde Subklinik Mastitis Tanısında Akut Faz Proteinlerinden Amiloid A ve C-Reaktif Protein Düzeylerinin Değerlendirilmesi

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