ELISA Kit DIY Materials for Superoxide Dismutase 1 (SOD1) Sus scrofa; Porcine (Pig) Assay Kit DIY Materials

ALS; ALS1; IPOA; Superoxide Dismutase 1, Soluble; Amyotrophic Lateral Sclerosis 1,Adult; Superoxide dismutase [Cu-Zn]

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity2.51ng/mL
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  • ELISA Kit DIY Materials for Superoxide Dismutase 1 (SOD1) Packages (Simulation)
  • ELISA Kit DIY Materials for Superoxide Dismutase 1 (SOD1) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Superoxide Dismutase 1, Soluble (SOD1).No significant cross-reactivity or interference between Superoxide Dismutase 1, Soluble (SOD1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Consequences of age on ischemic wound healing in rats: altered antioxidant activity and delayed wound closurePubmed:24443098
  • Diabetic cardiomyopathy is associated with defective myocellular copper regulation and both defects are rectified by divalent copper chelationPubmed:24927960
  • Antioxidant profile, carbonyl and lipid oxidation markers in the parotid and submandibular glands of rats in different periods of streptozotocin induced diabetesPubMed: 26143097
  • BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated RatsJournals: Omcl
  • КЛИНИЧЕСКОЕ ЗНАЧЕНИЕ ФАКТОРОВ КЛЕТОЧНОЙ РЕГУЛЯЦИИ И ФЕРМЕНТОВ АНТИОКСИДАНТНОЙ СИСТЕМЫ У ПАЦИЕНТОК РЕПРОДУКТИВНОГО ВОЗРАСТА С НАРУЖНЫМ ГЕНИТАЛЬНЫМ ЭНДОМЕТРИdocs:225188
  • Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariaepmc:PMC4794592
  • DDAH1 deficiency promotes intracellular oxidative stress and cell apoptosis via a miR-21-dependent pathway in mouse embryonic fibroblasts.Pubmed:26806551
  • Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects.pubmed:28242484
  • Clinical Characterization of Serum Docosahexaenoic Acid and Its Relationship With Inflammation Factors in Patients With Diabetic NephropathyPubmed:29507271
  • Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed ratsDoi: 10.1016/j.joim.2018.12.002
  • Restoration of myocellular copper-trafficking proteins and mitochondrial copper enzymes repairs cardiac function in rats with diabetes-evoked heart failurePubmed: 31821401
  • Grape seed extract improved the fertility-enhancing effect of atorvastatin in high-fat diet-induced testicular injury in rats: involvement of antioxidant and anti-apoptotic?¡­33793875
  • SOD1 is a Possible Predictor of COVID-19 Progression as Revealed by Plasma Proteomics34250342
  • Improved motility in the gastrointestinal tract of a postoperative ileus rat model with ilaprazole34697261
  • Serum Total SOD Activity and SOD1/2 Concentrations in Predicting All-Cause Mortality in Lung Cancer Patients34832849

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