ELISA Kit DIY Materials for Transferrin (TF)
TRF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
- UOM
- FOB US$ 958.00 US$ 1,596.00 US$ 2,873.00 US$ 5,586.00 US$ 9,576.00
- Quantity
Overview
Properties
- Product No.KSC036Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryTumor immunityInfection immunityImmune molecule
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.144ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Transferrin (TRF).No significant cross-reactivity or interference between Transferrin (TRF) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Identification of Altered Plasma Proteins by Proteomic Study in Valvular Heart Diseases and the Potential Clinical SignificancePubMed: PMC3754973
- The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Diseasepubmed:27782743
- Putative salivary biomarkers useful to differentiate patients with fibromyalgiaPubmed:29654921
- Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese PatientsPubmed: 30486327
- Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria
- New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemiaPubmed: 30792208