ELISA Kit DIY Materials for Trefoil Factor 2 (TFF2)

SML1; SP; Spasmolytic Protein 1; Spasmolysin; Spasmolytic polypeptide

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,166.00 US$ 1,944.00 US$ 3,499.00 US$ 6,804.00 US$ 11,664.00
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Overview

Properties

Product No.KSA748Mu01
Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryTumor immunity Gastroenterology Dermatology

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.156-10ng/mL
Applicable Sensitivity0.065ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Trefoil Factor 2 (TFF2). No significant cross-reactivity or interference between Trefoil Factor 2 (TFF2) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in ChinaBiomedcentral: Source
Obstructive sleep apnea and rhonchopathy are associated with downregulation of trefoil factor family peptide 3 (TFF3)-Implications of changes in oral mucus composition.pubmed:29028798
Exocrine tissue-driven TFF2 prevents apoptotic cell death of endocrine lineage during pancreas organogenesisPubmed: 30733468
ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanismPubmed: 31278375
Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid …
Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨¹ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und?¡