ELISA Kit DIY Materials for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,368.00 US$ 2,280.00 US$ 4,104.00 US$ 7,980.00 US$ 13,680.00
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Overview

Properties

Product No.KSG945Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only
Downloadn/a
CategoryEnzyme & Kinase

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range0.78-50ng/mL
Applicable Sensitivity0.25ng/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). No significant cross-reactivity or interference between Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesionsPubMed: 26232084
Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcomePubmed:26810533
Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy mnstemps:135
Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytespubmed:28667290
Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disabilityPubmed:29523900
Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injuryarticle:10.1088
Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhageISBN:978-952-03-0750-9
Association of neuronal repair biomarkers with delirium among survivors of critical illnessPubmed: 31896448
BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head TraumaPubmed: 32987792