ELISA Kit DIY Materials for Vascular Endothelial Growth Factor C (VEGFC)

VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,094.00 US$ 1,824.00 US$ 3,283.00 US$ 6,384.00 US$ 10,944.00
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Overview

Properties

Product No.KSA145Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only
Downloadn/a
CategoryCytokine Tumor immunity

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range15.6-1000pg/mL
Applicable Sensitivity6.6pg/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor C (VEGFC).No significant cross-reactivity or interference between Vascular Endothelial Growth Factor C (VEGFC) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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ELISA / CLIA Experiment Service

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Citations

Alternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinomaPubMed: 19241443
Expression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stagesPubMed: 26125824
Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxiaPubMed: 26622498
IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via MacrophagesPubMed: 26608451
Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells.pubmed:27383632
RNAi-mediated gene silencing of vascular endothelial growth factor C suppresses growth andinduces apoptosis in mouse breast cancer in vitro and in vivo.pubmed:27895746
Characterization of isolated liver sinusoidal endothelial cells for liver bioengineeringPubmed:29582235
Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cellsPubmed:29328465
Dynamic signature of lymphangiogenesis during acute kidney injury and chronic kidney diseasePubmed: 31019289
Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions?Pubmed: 32722957