ELISA Kit DIY Materials for 5-Hydroxytryptamine (5-HT) Pan-species (General) Assay Kit DIY Materials

5HT; Serotonin; N-Methyl-Gamma

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range11.11-900ng/mL
  • Applicable Sensitivity4.38ng/mL
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  • ELISA Kit DIY Materials for 5-Hydroxytryptamine (5-HT) Packages (Simulation)
  • ELISA Kit DIY Materials for 5-Hydroxytryptamine (5-HT) Packages (Simulation)
  • KSA808Ge11.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine (5-HT). No significant cross-reactivity or interference between 5-Hydroxytryptamine (5-HT) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Ginsenoside rich fraction of Panax ginseng C.A. Meyer improve feeding behavior following radiation-induced pica in ratsScienceDirect: S0367326X12001153
  • Sensitization induces hypersensitivity in trigeminal nervePubMed: 23106664
  • Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in ratsPubMed: 26684638
  • Catha edulis chewing effects on treatment of paranoid schizophrenic patientsPubMed: 25926735
  • Electroacupuncture reCavia (Guinea pig )late hypothalamic–pituitary–adrenal axis and enhance hippocampal serotonin system in a rat model of depressionPubmed:26773866
  • Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPEPubmed:27196063
  • Metabolic Features of Heart Failure with Different Etiology ofsp11075
  • Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migrainepubmed:27618395
  • PI3K/AKT signaling pathway activation in a rat model of migraine.pubmed:28791398
  • The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic …Pubmed:29379286
  • Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile AcidsPubmed:29853946
  • Changes in sexual performance and biochemical characterisation of functional neural regions: A study in serotonin transporter knockout male ratsPubmed: 31037750
  • Serotonin-Mediated Cardiac Analgesia Via Ah-Type Baroreceptor Activation Contributes to Silent Angina and Asymptomatic InfarctionPubmed: 31153963
  • TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway InflammationPubmed: 32117301
  • Neurotransmitter system aberrations in patients with drug addictionPubmed: 32804296
  • 重组人脑利钠肽治疗慢性肺心病急性加重期的临床效果及对5-HT 浓度的影响
  • Ganjiang granule regulates cecal microflora and serum biochemical components in a rat model of constipation-predominant irritable bowel syndrome
  • Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­33762821
  • O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress33651993
  • Investigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball players34090322
  • A specific primed immune response in red palm weevil, Rhynchophorus ferrugineus, is mediated by hemocyte differentiation and phagocytosisPubmed:35245605
  • Correlation between intestinal microbiotal imbalance and 5‐HT metabolism, immune inflammation in chronic unpredictable mild stress male ratsPubmed:35535862

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