ELISA Kit DIY Materials for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) 
ADMP-2; Aggrecanase-2
- UOM
- FOB US$ 1,260.00 US$ 2,100.00 US$ 3,780.00 US$ 7,350.00 US$ 12,600.00
- Quantity
Overview
Properties
- Product No.KSK205Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryEnzyme & Kinase
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.112ng/mL
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5). No significant cross-reactivity or interference between A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
- Lack of anti-inflammatory and anti-catabolic effects on basal inflamed osteoarthritic chondrocytes or synoviocytes by adipose stem cell-conditioned mediumPubMed: 26521751
- VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblastsPubmed:26818776
- From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.Pubmed:26991748
- Healthy and Osteoarthritic Synovial Fibroblasts Produce a Disintegrin and Metalloproteinase with Thrombospondin Motifs 4, 5, 7, and 12: Induction by IL-1β and Fibronectin and Contribution to Cartilage Damage.pubmed:27449198
- Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders.pubmed:28207788
- Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders10.1371:journal.pone.0172181
- From varices to venous ulceration: the story of chronic venous disease described by metalloproteinasespubmed:26991748
- Hemoglobin stimulates the expression of ADAMTS-5 and ADAMTS-9 by synovial cells: a possible cause of articular cartilage damage after intra-articular hemorrhage.pubmed:29137610
- The Effects of TNF-α Inhibition on the Metabolism of Cartilage: Relationship between KS, HA, HAPLN1 and ADAMTS4, ADAMTS5, TOS and TGF-β1 Plasma …Pubmed:35407621