ELISA Kit DIY Materials for Adenosine Triphosphate (ATP) Pan-species (General) Assay Kit DIY Materials

Adenosine-5'-Triphosphate

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1,000ng/mL
  • Applicable Sensitivity
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  • ELISA Kit DIY Materials for Adenosine Triphosphate (ATP) Packages (Simulation)
  • ELISA Kit DIY Materials for Adenosine Triphosphate (ATP) Packages (Simulation)
  • KSA349Ge11.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Adenosine Triphosphate (ATP). No significant cross-reactivity or interference between Adenosine Triphosphate (ATP) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female RatsIjvst: Source
  • Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathwaysPubMed: 25752913
  • Metabolic Features of Heart Failure with Different Etiology ofsp11075
  • Rifampicin-induced injury in L02 cells is alleviated by 4-PBA via inhibition of the PERK-ATF4-CHOP pathway.pubmed:27470132
  • Rifampicin-induced injury in HepG2 cells is alleviated by TUDCA via increasing bile acid transporters expression and enhancing the Nrf2-mediated adaptive response.pubmed:28688954
  • Chemiluminescent bioanalytical assays for clinical biomarkers2445/116053
  • The Antioxidative Action of ZTP by Increasing Nrf2/ARE Signal Pathway
  • Antihypoxic and anti-ischemic properties of the North Caucasus flora plant extracts
  • Effects of nanoparticles on Neuroinflammation in a Mouse Model of AsthmaPubmed: 31542455
  • Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling …Pubmed: 33179848
  • Ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate improve mitochondrial function under condition of cerebral ischemia in rat
  • Prostaglandin E receptor subtype 4 protects against diabetic cardiomyopathy by modulating cardiac fatty acid metabolism via FOXO1/CD36 signalling33647796
  • Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals ¡­
  • Estrogen inhibits bladder overactivity in rats with cyclophosphamide‐induced cystitis via downregulating the expression of P2X3 receptors in bladder epithelium cells34622458
  • A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating …Pubmed:35281603

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