ELISA Kit DIY Materials for Adrenomedullin (ADM)

AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,134.00 US$ 1,890.00 US$ 3,402.00 US$ 6,615.00 US$ 11,340.00
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Overview

Properties

Product No.KSA220Hu11
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only
Downloadn/a
CategoryEndocrinology Hormone metabolism

Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
Detectable Range12.35-1000pg/mL
Applicable Sensitivity4.33pg/mL

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Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Adrenomedullin (ADM). No significant cross-reactivity or interference between Adrenomedullin (ADM) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Citations

Gingival crevicular fluid adrenomedullin level in individuals with and without diabetes mellitus type 2Wiley: Source
Comparison of peri-implant crevicular fluid levels of adrenomedullin and human beta defensins 1 and 2 from mandibular implants with different implant stability quotient levels in nonsmoker patientsPubmed: 23898869
Short-term hypoxia/reoxygenation activates the angiogenic pathway in rat caudate putamen.Pubmed: 23660671
DNA methylation patterns in newborns exposed to tobacco in uteroPubmed:25623364
Adrenomedullin—A New Marker in Febrile Neutropenia: Comparison With CRP and ProcalcitoninPubMed: 26271020
Angiogenesis is VEGF-independent in the aged striatum of male rats exposed to acute hypoxia.pubmed:28501895
Adrenomedullin and Galanin Responses to Orthostasis in Older Persons pubmed:28796366
ADRENOMEDULIN DI KARSINOMA PAYUDARA DENGAN METASTASIS:
Composition and cardioprotective effects of Primula veris L. solid herbal extract in experimental chronic heart failureDoi: 10.1016/j.phymed.2018.09.015
Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
Clinical and experimental investigation of the nervous system of neonatal foals. Neuroendocrin transition from foetal consciousness to perinatal life
Hemodynamic Responses in Lower Limb Lymphedema Patients Undergoing Physical Therapy34356497