ELISA Kit DIY Materials for Advanced Glycation End Product (AGE) Pan-species (General) Assay Kit DIY Materials

AGEs; Advanced Glycation End Products

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Overview
Properties
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range98.77-8,000ng/m
  • Applicable Sensitivity38.2ng/mL
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  • ELISA Kit DIY Materials for Advanced Glycation End Product (AGE) Packages (Simulation)
  • ELISA Kit DIY Materials for Advanced Glycation End Product (AGE) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Advanced Glycation End Product (AGE). No significant cross-reactivity or interference between Advanced Glycation End Product (AGE) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Threshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM)PubMed: S0198885912005642
  • A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing processPubMed: 22017743
  • Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D.Pubmed: 23829785
  • Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients.Pubmed: 23721855
  • Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus.Pubmed:24397392
  • Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complicationsPubmed:24984291
  • Nitroxides prevent protein glycoxidation in vitro.Pubmed:25363554
  • Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apneaPubmed:25566941
  • Ascorbic acid and protein glycationPubMed: 26163454
  • Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazonePubMed: 26321377
  • Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apneaPubMed: 25994997
  • Glycation of bovine serum albumin by ascorbatePubMed: 26202868
  • Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human salivaPubMed: 26608886
  • Inhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats.PubMed: 26207852
  • ADVANCED GLYCATION END PRODUCTS'RESPONSE TO RESISTANCE TRAINING IN POSTMENOPAUSAL WOMEN WITH TYPE II DIABETES140251:
  • Effects of edible bird's nest on hippocampal and cortical neurodegeneration in ovariectomized ratsPubMed: 25920003
  • Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cellsPubmed:26952874
  • Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung InjuryPubmed:27334732
  • The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass VeinPubmed:26905625
  • Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy.pubmed:28087385
  • Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine.pubmed:28214842
  • Glutathione is the main endogenous inhibitor of protein glycation.pubmed:28218612
  • Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone.pubmed:26321377
  • Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathwaypubmed:29097668
  • Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairmentpubmed:29138799
  • (台灣農業研究67 (4): 355-364) 香菇柄水萃物改善糖尿病降低阿茲海默氏症罹病風險初探
  • Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis PatientsPubmed: 30847765
  • Cellular Metabolomics Reveal the Mechanism Underlying the Anti-Atherosclerotic Effects of Aspirin Eugenol Ester on Vascular Endothelial DysfunctionPubmed: 31261711
  • Fushiming Capsule Attenuates Diabetic Rat Retina Damage via Antioxidation and Anti-Inflammation
  • Effect of Advanced Glycation End Products on the Progression of Alzheimer's DiseasePubmed: 31561370
  • Protective effect of vanillin on diabetic nephropathy by decreasing advanced glycation end products in ratsPubmed: 31759039
  • Supplementation with Korean Red Ginseng Improves Current Perception Threshold in Korean Type 2 Diabetes Patients: A Randomized, Double-Blind, Placebo …Pubmed: 32025522
  • A New Insight into Meloxicam: Assessment of Antioxidant and Anti-Glycating Activity in In Vitro StudiesPubmed: 32927809
  • Advanced glycation end products enhance macrophage polarization to the M1 phenotype via the HIF-1α/PDK4 pathwayPubmed: 32464167
  • The effect of Korean Red Ginseng on sarcopenia biomarkers in type 2 diabetes patientsPubmed: 32470863
  • The Role of PPARγ in Hyperglycemia-induced Deleterious Effect on Chondrocytes
  • Young and Undamaged rMSA Improves the Healthspan and Lifespan of Mice34439857
  • Dietary Advanced Glycation End Products Shift the Gut Microbiota Composition and Induce Insulin Resistance in MicePubmed:35210793

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