ELISA Kit DIY Materials for Aggrecan (AGC)

ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1

UOM
1. 96T*5
2. 96T*10
3. 96T*20
4. 96T*50
5. 96T*100
FOB US$ 1,197.00 US$ 1,995.00 US$ 3,591.00 US$ 6,983.00 US$ 11,970.00
Quantity

Add to Cart Distributors

Overview

Properties

Product No.KSB908Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
Research use only
DownloadInstruction Manual
CategorySignal transduction Metabolic pathway Bone metabolism

Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
Detectable Range1.56-100ng/mL
Applicable Sensitivity0.59ng/mL

Share your citation Upload your experimental result Review Leave a message

Sign into your account

Email *

Password *

Verification code*

Create a new account

Share a new citation as an author

Title *

Journal

Link*

Upload your experimental result

Application*

Experimental result*

Review

Rating

Serial No* Please attach serial No. on instruction manual

Contact us
Please fill in the blank.

Name*

Organization

Address

E-mail address*

Telephone

Inquiry*

Verification code* CheckCode

Packages (Simulation)
Packages (Simulation)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Aggrecan (AGC). No significant cross-reactivity or interference between Aggrecan (AGC) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Giveaways

ELISA / CLIA Experiment Service

Increment services

Citations

Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cellsRsmjournals: Source
In vitro tenocyte metabolism in aging and oestrogen deficiencySpringer: Source
A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic ApproachesPlosone: Source
Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly.Pubmed: 24739658
Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of compositionScienceDirect: S0928493113006656
Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented mediaPubmed:Pmc4130592
Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytesPubmed:25333747
Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage RegenerationPubMed: 25787871
Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigsPubmed:26802547
Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytesPubmed:27090063
Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis.pubmed:28187731
Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α InhibitorsPubmed: 32392807
Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs
Aggrecan-a new biomarker for acute type A aortic dissection33990642